Neoplasma Vol.66, No.2, p.203-210, 2019
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Title: Skullcapflavone I inhibits proliferation of human colorectal cancer cells via down-regulation of miR-107 expression |
Author: W. ZHANG, W. Li, X. HAN |
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Abstract: Colorectal cancer (CRC) is a common malignant tumor with high global increase and mortality. While Skullcapflavone I has been reported to exert anti-tumor effect in several cancers, its role in CRC has not previously been investigated. Recent studies have also demonstrated that microRNA-107 (miR-107) and tropomyosin alpha-1 (TPM1) are important regulators of cancer cell proliferation, but it remains unclear if these are involved in regulating the effect of Skullcapflavone I on CRC cells. This study therefore assessed the effects of Skullcapflavone I on CRC cell proliferation and investigated miR-107 and TPM1 regulatory effects on this process. The results showed that Skullcapflavone I significantly suppressed cell proliferation and viability and down-regulated PCNA and Cyclin D1protein levels. It also down-regulated miR-107 expression which then promoted TPM1 expression, but miR-107 over-expression abolished Skullcapflavone I anti-proliferative effects. Furthermore, Skullcapflavone I inhibited the activations of MEK/ERK and NF-κB signal pathway activation by regulating TPM1 in HCT116 cells. These results demonstrated that Skullcapflavone I increased the expression of TPM1 by down-regulating miR-107 and inhibiting the MEK/ERK and NF-κB signal pathways. It then inhibited HCT116 cell proliferation, and therefore Skullcapflavone I may provide new methodology in colorectal cancer treatment.
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Keywords: Skullcapflavone I, proliferation, microRNA-107, TPM1, MEK/ERK, NF-κB |
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Published online: 19-Mar-2019
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Year: 2019, Volume: 66, Issue: 2 |
Page From: 203, Page To: 210 |
doi:10.4149/neo_2018_180427N279
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