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Acta Virologica Vol.53, No.3, p.203-206, 2009 |
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Title: Inhibition of Bovine herpesvirus multiplication in MDBK cells by small interfering RNAs | ||
Author: P. S. Narute, A. A. Raut, M. Saini, A. Rai, P. K. Gupta | ||
Abstract: Small interfering RNA (siRNA) mediated gene silencing is a promising approach in antiviral therapy. To investigate the antiviral effects of siRNAs on Bovine herpesvirus 1 (BoHV-1) multiplication, we designed and in vitro synthesized two siRNAs (siRNA-1 and siRNA-2) targeting the UL25 gene that is essential for BHV-1 multiplication. siRNA-1 and siRNA-2 inhibited the BoHV-1 multiplication in MDBK cells to a different extent, namely by 11% and 40%, respectively, as demonstrated by virus titers (TCID50/ml) determined in cell culture. This indicates that, in general, siRNAs can inhibit BHV-1 multiplication in vitro and could be used also against a BHV-1 infection in vivo following optimization of their application. |
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Keywords: siRNAs; Bovine herpesvirus 1; UL25 gene | ||
Year: 2009, Volume: 53, Issue: 3 | Page From: 203, Page To: 206 | |
doi:10.4149/av_2009_03_203 |
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