Journal info
|
||
Select Journal
Journals
Bratislava Medical Journal Endocrine Regulations General Physiology and Biophysics Neoplasma 2024 Ahead of print 2023 2022 2021 2020 2019 2018 2017 2016 2015 2014 2013 2012 2011 2010 2009 2008 2007 2006 2005 2004 2003 Acta Virologica Studia Psychologica Cardiology Letters Psychológia a patopsych. dieťaťa Kovove Materialy-Metallic Materials Slovenská hudbaWebshop Cart
Your Cart is currently empty.
Info: Your browser does not accept cookies. To put products into your cart and purchase them you need to enable cookies.
Neoplasma Vol.53, p.518-523, 2006 |
||
Title: Constitutive NF-kappaB activity in colorectal cancer cells: impact on radiation-induced NF-kappaB activity, radiosensitivity, and apoptosis | ||
Author: R. VOBORIL, J. WEBEROVA-VOBORILOVA | ||
Abstract: Constitutive NF-κB activity has been found in many cancer cells of different origin. In our study we focused on constitutive NF-κB activity and its impact on radiation-induced NF-κB activity, intrinsic radiosensitivity, and apoptosis. Using colorectal cancer cell lines (Caco-2, SW480, SW620) we demonstrated that each cell line expresses different level of constitutive NF-κB activity. Moreover, irradiation caused secondary NF-κB activation which differed in each cell line. The cell lines tested displayed also different intrinsic radiosensitivities as was determined by clonogenic assay, and different spontaneous and radiation-induced apoptosis determined by activity of caspase 3. Complex analysis of our results revealed that there was a strong correlation between constitutive NF-κB activity and radiation-induced NF-κB activity (r=0.835), the level of constitutive NF-κB activity predicted the level of secondary, radiation-induced NF-κB activity. Furthermore, SW620 cells with the highest level of constitutive NF-κB activity displayed the lowest radiosensitivity and the lowest level
of spontaneous apoptosis; Caco-2 cells with almost undetectable level of constitutive NF-κB activity displayed the highest radiosensitivity and even highest level of spontaneous apoptosis. SW480 cells showed intermediate level of constitutive NF-κB activity, intermediate radiosensitivity and intermediate level of spontaneous apoptosis. Our data suggest that the
level of constitutive NF-κB activity may predict radiosensitivity of colorectal cancer cells. Such prediction may allow the individualization of patient treatment by radiotherapy. |
||
Keywords: NF-kappaB, colorectal cancer, prediction, radiosensitivity, apoptosis | ||
Year: 2006, Volume: 53, Issue: | Page From: 518, Page To: 523 | |
|
download file |
|