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Neoplasma Vol.51, p.481-486, 2004 |
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Title: Early nuclear alterations and immunohistochemical expression of Ki- 67, Erb-B2, vascular endothelial growth factor (VEGF), transforming growth factor (TGF-BETA 1) and integrine-linked kinase (ILK) two days after tamoxifen in breast carcinoma | ||
Author: A.M.L., MORENA ; C.T.F., OSHIMA ; L.H., GEBRIM ; M.I., EGAMI ; R.A., SEGRETO ; V.P.C., TEIXEIRA ; | ||
Abstract: The purpose of the present study was to evaluate breast carcinoma
samples before and two days after treatment with tamoxifen in
order to analyse early histopathological alterations --
particulary nuclear alterations -- as well as immunohistochemical
expression of Ki-67, Erb-B2, VEGF, TGf-beta1 and ILK proteins.
Twenty one cases of invasive ductal and lobular breast carcinoma
were studied. Patients were submitted to biopsy of the lesion and,
after confirmation of the diagnosis, they received 20 mg of
tamoxifen a day, beginning two days before surgery. The samples
obtained during biopsy and after surgery were stained with HE for
histopathological diagnosis. Estrogen receptor was positive in 18
cases and negative in 3. The immunohistochemical method was
applied for the detection of Ki-67, Erb-B2, protein, vascular
endothelial growth factor (VEGF), transforming growth factor beta
(TGF-beta1) and integrin linked kinase (ILK).
Two days after tamoxifen treatment, the following results were
observed: 1) decrease in the cell volume, chomatine condensation,
nucleoli less evident and clearly defined nuclear limits; 2)
significant reduction in the expression of Erb-B2 protein and
significant increase in the expression of TGF-beta1 protein; 3)
expression of others proteins (Ki-67, VEGF and ILK) was not
altered during the indicated time frame.
Our results suggest that analyzing nuclear alterations and
expression of Erb-B2 and TGF-beta1 proteins would be useful
to assess the initial response to tamoxifen.
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Keywords: breast cancer, tamoxifen, immunohistochemistry, tumor markers, Erb- B2, TGF-beta 1 | ||
Year: 2004, Volume: 51, Issue: | Page From: 481, Page To: 486 | |
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