Journal info
|
||
Select Journal
Journals
Bratislava Medical Journal Endocrine Regulations General Physiology and Biophysics Neoplasma 2024 Ahead of print 2023 2022 2021 2020 2019 2018 2017 2016 2015 2014 2013 2012 2011 2010 2009 2008 2007 2006 2005 2004 2003 Acta Virologica Studia Psychologica Cardiology Letters Psychológia a patopsych. dieťaťa Kovove Materialy-Metallic Materials Slovenská hudbaWebshop Cart
Your Cart is currently empty.
Info: Your browser does not accept cookies. To put products into your cart and purchase them you need to enable cookies.
Neoplasma Vol.51, p.395-399, 2004 |
||
Title: Determination of free radical scavenging activity of quercetin, rutin, luteolin and apigenin in H2O2-treated human ML cells K562 | ||
Author: L., NOVOTNY ; A, VACHALKOVA ; K., HORVATHOVA ; D., TOTHOVA ; | ||
Abstract: We investigated protective effects of four flavonoids against H2O2-
induced DNA damage in human myelogenous leukemia cells (K562)
using the comet assay. The structural difference of studied
flavonoids -- quercetin, rutin, luteolin and apigenin -- are
characterized by the number of hydroxyl groups on the B ring. The
presence of an o-dihydroxy structure on the B-ring confers a
higher degree of stability to the flavonoid phenoxyl radicals by
participating in electron delocalization and is, therefore, an
important determinant for antioxidative potential. The results
correlate with earlier published data obtained in murine leukemia
cell line L1210. Hydrogen peroxide induced in human K562 cells a
concentration-dependent increase of single cell DNA strand breaks.
The strongest inhibition against H2O2-induced DNA damage (44%,
42%) was found in a range of luteolin and quercetin concentrations
of 20--100 Ţmol/l. Protective effect of rutin (100 and 1000
Ţmol/l) was only marginal (8--10%). Apigenin had no protective
effect on DNA single strand breaks induced by H2O2. Luteolin and
quercetin are therefore effective in the protection of human
single cell DNA from oxidative attack.
|
||
Keywords: quercetin, rutin, apigenin, luteolin, cytotoxicity, comet assay | ||
Year: 2004, Volume: 51, Issue: | Page From: 395, Page To: 399 | |
|
download file |
|