Neoplasma Vol.52, p.260-266, 2005
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Title: Effect of testosterone on growth of P388 leukemia cell line in
vivo and in vitro.
Distribution of peripheral blood T lymphocytes and cell cycle
progression
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Author: S., ABOUDKHIL
; L., HENRY
; A., ZAID
; J.P., BUREAU
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Abstract: In transplanted mice, the P388 tumor grew better in castrated than
in non castrated (NC) mice. The proportion of CD8+ in
the blood was more numerous in NC mice. The T cell subsets (CD4+
and CD8+) were also high in the mice with small tumor
tissue (<10 mg). The correlation observed between the tumor weight
and T cell subset in PBL and in the mice with small tumors
could confirm the important intervention of CD4+ and CD8+ cells to
inhibit growth of tumor.
Depo-testosterone (DT) injection reduced strongly weight and tumor
growth in mice. On top of that, DT administration
induced a significant increase in the percentage of blood CD8+
cells in grafted mice.
The effect of DT was studied on the cell cycle progression, in
tumor tissue of P388 tumor bearing BDF1 mice and in P388
murine leukemia cell line in culture. The cell cycle analysis in
tumor tissue showed that DT decreased both the cells in S
phase and the proliferating leukemic cells, with accumulation of
cells in G0/G1 phase. The testosterone can inhibit the
proliferation
of leukemic cells with a pharmacological dose (10-7 M). This
growth inhibition, dose and time dependent, was associated
with cell cycle arrest; P388 cells accumulates in G0/G1 phase. We
also observed a correlation between tumor
weight and the percentage of cells in G0/G1 and the relative
number of cells in proliferative state (S + G2/M).
To conclude, our experiments reported that testosterone prevents
the growth of tumor: indirectly by modulation of subsets
T cells distribution and directly by the alteration of the cell
cycle.
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Keywords: testosterone, P388 leukemia cell line, peripheral blood
lymphocyte, tumor tissue, subsets T cells, cell cycle.
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Year: 2005, Volume: 52, Issue: |
Page From: 260, Page To: 266 |
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