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Neoplasma Vol.51, p.415-421, 2004 |
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Title: Semi-quantitative RT-PCR assessment of molecular markers in breast large-core needle biopsies | ||
Author: K., POHLODEK ; Š., GALBAVY ; M., BARTOSOVA ; V., MUCHA ; K., HOLOMAN ; J., PASTOREK ; S., PASTOREKOVA ; | ||
Abstract: Large-core needle biopsies are frequently used for the
preoperative evaluation of the breast lesions. In addition to
initial diagnostic information, they can show the status of
molecular markers with predictive and prognostic value and further
contribute to an optimal selection of treatment strategy. So far,
the potential use of large-core needle biopsies in assessment of
marker profile of the breast lesions was studied using the
immunostaining approaches. In this work, we sought to determine
whether analysis of the large-core needle biopsy by semi-
quantitative reverse-transcription polymerase chain reaction
reveals molecular data that correspond with the marker status of
the subsequently removed tumor. Five molecular markers including
ER, PR, c-erbB-2/HER-2/neu, c-erbB-3/HER-3, and CD44 were assessed
on mRNA isolated from 23 large-core needle biopsies and
corresponding surgical breast cancer specimens using ß2-
microglobulin as an internal standard. Significant or highly
significant correlation between core biopsies and excised tumors
was observed for each marker when the mRNA expression status was
scored as positive or negative, with the concordance of the data
ranging from 73.9% to 86%. Using the dichotomous scoring, majority
of the biopsies (75%) displayed molecular profile that was either
identical to the profile of the related tumor specimen, or with
the difference in one marker. However, no significant correlation
was found when the levels of the markers were expressed as
continuous variables, possibly due to intratumoral cell
heterogeneity. These results suggest potential usefulness and
reliability of semi-quantitative RT PCR in the evaluation of large-
core needle biopsies with regard to marker positivity or
negativity. On the other hand, the marker-related data expressed
as continuous variables cannot be accurately assessed on the large-
core needle specimens using this approach, indicating the need for
methodological improvements.
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Keywords: large-core needle biopsy, semi-quantitative reverse transcription PCR, breast cancer, molecular markers | ||
Year: 2004, Volume: 51, Issue: | Page From: 415, Page To: 421 | |
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