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Acta Virologica Vol.48, p.115-121, 2004 |
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Title: GENETIC STABILITY OF THE ATTACHMENT GLYCOPROTEIN OF HUMAN RESPIRATORY SYNCYTIAL VIRUSES DURING SERIAL PASSAGES IN CELL CULTURES | ||
Author: M. DE SIERRA, J. ARBIZA | ||
Abstract: Thirteen isolates of human respiratory syncytial viruses (HRSV) of groups A and B were isolated in HEp-2 cells from nasopharyngeal aspirates (NPA) from the children with acute respiratory infections. Three isolates of HRSV of group A were propagated in HEp-2 cells in 20 serial passages. Nucleotide sequences of the products obtained by RT-PCR from the glycoprotein (G) hypervariable region of the original virus isolates in NPA and those after one or several passages were compared. All the isolates analyzed showed no changes during passaging in HEp-2 cells. |
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Keywords: human respiratory syncytial viruses; genetic stability; attachment glycoprotein | ||
Year: 2004, Volume: 48, Issue: | Page From: 115, Page To: 121 | |
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